IDENTIFIKASI Mycobacterium bovis DENGAN KONSENTRASI DNA BERTINGKAT MENGGUNAKAN TEKNIK POLYMERASE CHAIN REACTION (PCR)

Main Authors: , DITA DHARMAYANTI, , Prof. Dr. drh. Ida Tjahajati, M.P.
Format: Thesis NonPeerReviewed
Terbitan: [Yogyakarta] : Universitas Gadjah Mada , 2013
Subjects:
ETD
Online Access: https://repository.ugm.ac.id/121146/
http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=61203
Daftar Isi:
  • Mycobacterium sp. is a bacteria that can cause tuberculosis disease in domestic animals. Mycobacterium bovis pathogens in 3rd risk groups and Indonesia as the country's fourth-largest contributor of Tuberculosis in the world after India, China and South Africa. Based on these facts, the research was conducted to identify M. bovis with DNA stage concentrations, hope of the lowest concentration can be used as a reference for the detection of tuberculosis. Isolate DNA samples obtained from the Center for Veterinary Research (BBalitvet) in Bogor, West Java. Dilution of the DNA was started from concentration 5000 pg / mL