UJI SENSITIVITAS BAKTERI Staphylococcus aureus PADA SUSU MASTITIS SUBKLINIS TERHADAP CEFIXIME DAN PIPERACILLIN DI KELOMPOK TERNAK SARONO MAKMUR WUKIRSARI, CANGKRINGAN, SLEMAN, YOGYAKARTA
| Main Authors: | , OPTI YASHINTA CHATRA, , Dr. drh. Yanuartono, M.P. |
|---|---|
| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
|
| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/121096/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=61150 |
Daftar Isi:
- Mastitis is an inflammation in the mammary gland. It may infect clinically or subclinically. One of the causal factors of subclinical mastitis is Staphylococcus aureus. This study aims to determine the sensitivity level of Staphylococcus aureus toward Cefixime and Piperacillin. Sixteen samples of milk have been through mastitis test using reagent CMT. The samples were inoculated in BHI and then were planted in blood plate and MacConkey Agar (MCA) with the incubation period was 24 hours in the temperature of 37 o C. The grown colonies were grouped based on the morphology. Biochemic test, Gram�s staining, catalase test, coagulase test, and DNase test were conducted to confirm what kind of bacteria that grew. To figure out the sensitivity level of the bacteria, the colonies growing on NA medium were diluted with NaCl, planted on Muller Hinton Agar (MHA), and then given the antibiotics using paper disc. The zone of inhibition was measured after the media was incubated for 24 hours in the temperature of 37 o C. The result of the isolation showed 2 of 16 samples (12.5%) were positive infected by Staphylococcus aureus. It can be concluded that the sensitivity level of Staphylococcus aureus toward piperacillin is 100% while its resistance level toward cefixime is 100%.