GAMBARAN LESI MIKROSKOPIK BERBAGAI ORGAN PADA BROILER YANG DITIMBULKAN OLEH PATOTIPE VELOGENIC NEUROTROPIC NEWCASTLE DISEASE VIRUS

Main Authors: , HANINDHITYA LINTANG A S, , Prof. Widya Asmara, S.U., Ph.D.
Format: Thesis NonPeerReviewed
Terbitan: [Yogyakarta] : Universitas Gadjah Mada , 2013
Subjects:
ETD
Online Access: https://repository.ugm.ac.id/121078/
http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=61130
Daftar Isi:
  • Newcastle disease is contagious avian caused by avian paramyxovirus type 1 (APMV-1). Based on its clinical symptoms, Newcastle disease is divided into 4 pathotype: velogenic viscerotropic Newcastle disease (VVND), velogenic neurotrophic Newcastle disease (VNND), mesogenic Newcastle disease and lentogenic Newcastle disease. The aim of this study is to determine the microscopic lesions in chicken which showed the symptoms of velogenic neurotropic Newcastle Disease (VNND). Specimen for histopathological examination was taken from the brain, servicalis vagal nerve branches, lungs, kidney, small intestine, ceca tonsil, and proventriculus. Virus isolation from lung, spleen, and cloaca swab were performed by inoculating suspected sample into 10- days old chicken embryo, which were specific antibody negative to NDV. For confirmatory diagnosis, virus detection was confirmed by hemagglutination test (HA) and hemaglutination inhibition test (HI). HA positive indicated the growth of virus which has ability to agglutinate chicken red blood cell. HI test using specific antibody was then applied to confirm the presence of NDV. Histopathological examination showed perivascular cuffing in the cerebrum and necrosis of mucosa of proventriculus. For confirmatory diagnosis, virus detection was confirmed by hemagglutination test (HA) and hemaglutination inhibition test (HI).