KAJIAN KERAGAMAN GENETIK GEN Cytochrome Oxidase SubUnit 1 (COX1) Tarsius sp. SEBAGAI UPAYA PENENTUAN ASAL USUL Tarsius sp
| Main Authors: | , Alnita Baaka, , Dr. drh. Rini Widayanti., MP. |
|---|---|
| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
|
| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/120906/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=60944 |
Daftar Isi:
- Tarsier is one of Indonesian wildlife animal that needs specific attention for their conservation. So far, conservation of Tarsius sp. can be done by either insitu and ex-situ breeding programs. To avoid interspecies hybrid, identification of tarsier species based on morphological and molecular characters is required. The purpose of the study is to obtain genetic diversity of cytochrome oxidase subunit 1 (COX1) gene for genetic markers to identify Tarsius sp. The DNA samples were obtained from muscle tissues of T.bancanus from Sumatra and Kalimantan, T.spectrum from North Sulawesi, and T.dianae from Central Sulawesi, kindly provided by Dr.drh. Rini Widayanti, MP., and were isolated using QIAamp ® DNA Tissue kit Qiagen production. Isolated DNA then were used as template in the PCR amplification process. Primer of COX1 gene were designed using primer3-Online program. COX1 gene then were amplified using PCR and the purified DNA results were sequenced. COX1 gene nucleotide sequence were aligned with other primates taken from GenBank using the Clustal W software. To find out the genetic distance, the data then were analyzed using the kimura 2 parameter models and phylogeny analysis using the Neighbour-Joining method with 1000 repetitions that exist in the program MEGA version 5.05. The results show that 239 nucleotides out of 1542 and 1545 and 21 amino acids out of 514 and 515 are diverse. Genetic distances of COX1 gene of Tarsius is between 0% to 19,1% with average 10,2%. The amino acid and nucleotide of COX1 gene is able to distinguishing T. bancanus, T.spectrum and T. dianae, but unable to distinguishing between T. bancanus from Kalimantan and Sumatra. From the whole analysis, we conclude that phylogenetically Tarsius sp. is belonging to the suborder Prosimii (Strepsirhini).