PERBANDINGAN EKSPRESI PROTEIN Bak DAN INDEKS APOPTOSIS SEL TROFOBLAS PADA KEHAMILAN PREEKLAMPSIA BERAT DAN KEHAMILAN NORMOTENSI
| Main Authors: | , Marihot Pasaribu, , dr. Diah Rumekti Hadiati, M.Sc SpOG(K) |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/119730/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=59733 |
Daftar Isi:
- Background: Placental is a central and important focus on the pathogenesis of preeclampsia/eclampsia. Failure of trophoblast cell to the spiral arteries remodeling process due to excessive apoptosis causes uteroplacenter ischemia and damage of endothelial cells that give rise to clinical manifestasions of preeclampsia/ eclampsia. Excessive trophoblast apoptosis in preeclampsia/eclampsia mainly occurs through the intrinsic pathway in which expression of Bak protein increases mitochondrial membranes permeability of cytochrome C which further activated the caspase cascade and become involved in the process of cell death. Objective of study: To compare the expression of Bak protein and trophoblastic apoptotic index between severe preeclampsia and normotensive pregnancies. Methods: Cross sectional study which consist of 43 severe preeclampsia pregnancies and 38 normotensive pregnancies recruited between October 2011- March 2012. Trophoblastic cells Bak protein expression is measured by imunohistochemical staining technique and trophoblast apoptotic index quantification is examined by TUNEL assay. Results: There are significant differences of Bak protein expression in placental tissue of preeclampsia group (2.37±0.91, p=0.012) compared by normotensive group (1.92�0.59). There are significant differences of apoptotic index in placental tissue of preeclampsia group (0.44 0 ± .26, p=0.00) compared by normotensive group (0.24�0.20). Conclusion: The Bak protein expression and the number of apoptotic cells in trophoblast tissue of preeclampsia complicated pregnancy were higher than normal pregnancy.