ISOLASI DAN IDENTIFIKASI SENYAWA PENANGKAP RADIKAL BEBAS 2,2-DIFENIL-1 PIKRILHIDRAZIL DARI DAUN KEDONDONG (Spondias dulcis Soland. ex Park.)
| Main Authors: | , RESLELY HARJANTI, , Prof. Dr. Wahyono, SU., Apt |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/118591/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=58566 |
Daftar Isi:
- Spondias dulcis Soland ex Park fruit has already known to be rich of antioxidants due to vitamin C and polyphenolic content but less known about the antioxidant potency of the leaves. The young leaves are consumed as a culinary dish by people in some areas in Indonesia. This study was aimed to reveal the antioxidant potency of S. dulcis leaves and to isolate and to identify the active compound by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method as a bioassay guidance. A total of 1500 g of S. dulcis dried powder was macerated in 96% ethanol, followed by solvent evaporation. Extract yielded was fractionated by using liquid-liquid partition on methanol-water (9:1)-hexane, and water-ethyl acetate, successively. The fraction were tested for the antioxidant potency by using spectrophotometric-DPPH assay. Fraction with the highest free-radical scavenging activity was further fractionated by using vacuum liquid chromatography with gradient polarity solvent as follows: n-hexane 100%, various proportion of n-hexane-ethyl acetate and ethyl acetate-methanol, and methanol 100%. Each sub fractions were tested qualitatively by using DPPH-autography. The most intensive spot was isolated by using preparative TLC. Purity of the isolate was determined by using TLC with three different polarity eluents. The isolate was then determined its free-radical scavenging activity by spectrophotometric-DPPH assay where quercetin was used as positive control. Isolateâ��s chemical structure was determined by using UV-Vis spectrophotometer, infra-red, 1D and 2D 1H-NMR, 13C-NMR spectroscopes and ESI-MS. Isolate showed high antioxidant potency with IC50 value of 10.78 Î1⁄4g/ mL (quercetin IC50 value was 1.71 Î1⁄4g/ mL). Result of spectroscopy data analyses identified the isolate chemical structure as rutin.