PENGEMBANGAN DAN VALIDASI METODE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY UNTUK PENETAPAN KADAR ATENOLOL DENGAN DERIVATISASI 1-FLUORO-2,4-DINITROBENZEN DALAM SPIKED PLASMA
| Main Authors: | , Luh Putu Mirah Kusuma Dewi, , Prof.Dr.Sudibyo Martono, M.S., Apt |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/118574/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=58549 |
Daftar Isi:
- Atenolol, 4-[2-hydroxy-3-[(1-methylethyl)amino]propoxy]- benzeneacetamide is a cardioselective Î21-adrenergic receptor blocking agent prescribed for the treatment of hypertension, angina pectoris, and cardiac arrhythmias. The present research is to develop and validate a new HPLC method with UV detection for quantitative determination of atenolol derivatized with 1- fluoro-2,4-dinitrobenzen (FDNB) in spiked human plasma. The proposed method was based on the reaction of atenolol with FDNB in borate buffer solution (pH 10,5) which formed a derivatized product with absorption at 249 nm. The product was separated by HPLC using Chromolith RP18e column (100 x 4,6 mm, 2 Î1⁄4m) as the stationary phase while the mobile phase consist acetat buffer solution (pH 3,5) â�� asetonitril (70 : 30 v/v), at flow rate 1,0 mL/min and UV detection at 249 nm. The developed method was validated by means of accuracy, precision, linearity, selectivity, LOD and LOQto fulfill the requirement for validity. The assay method was found to be linear from 100 to 800 ng/mL with limit of detection (LOD) 1 ng/mL and limit of quantitation (LOQ) 18,261 ng/mL. The recovery was 92,807 â�� 113,63 % (% RSD = 1,02 â�� 1,78%).