ISOLASI DAN IDENTIFIKASI SENYAWA PENANGKAP RADIKAL 2,2- DIFENIL-1-PIKRILHIDRAZIL PADA JAMUR PANGAN TERPILIH KOLEKSI DARI DAERAH ISTIMEWA YOGYAKARTA
| Main Authors: | , KHOIRUN NISA, , Prof. Dr. Subagus Wahyuono., Apt |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2012
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/100901/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=57736 |
Daftar Isi:
- Antioxidant is an important agent to protect and reduce the oxidative damage. The aim of the research is to isolate and to identify a 2,2-diphenyl-1- picrylhydrazyl radical scavenger compound of selected edible mushroom among 5 edible mushrooms in DIY. Isolation was also carried out to the main compound found in the selected mushroom. Antioxidant activity assay of 5 mushroom ethanolic extracts exhibited that ear mushroom (Auricularia polytricha (Mont.) Sacc.) has the highest antioxidant activity (IC50 49,3 ± 3,19 Î1⁄4g/ml). The ear mushroom extract was fractionated using 80 % methanol. The soluble fraction was separated using Vacum Liquid Chromatography with 4 eluents and obtained 4 fractions (F1-F4). Active fraction (F3) was isolated using Preparative TLC and resulted isolate 1. Isolation of main coumpound produced an isolate 2. Both of isolates were identied by UV, IR, GC/LC-MS dan 1H/13C-NMR spectroscopy. The result exhibited that IC50 of isolate 1 was 347,8 ± 3,09 Î1⁄4g/mL. The UV spectrum of isolate 1 showed λMeOH max at 203 nm. The IR spectrum of isolate 1 showed absorption of a hydroxy (3600 cm-1), double bond (1650 cm-1), CH2 (1400 cm-1) and CH3 (1250 cm-1). Analysis of 1H-NMR (500MHz, CDCl3) was: Î ́ [ppm] 0,87