PENGGUNAAN BAKTERI DAN JAMUR PENDEGRADASI LIGNOSELULOSA SALURAN PENCERNAAN HERBIVORA UNTUK MENINGKATKAN KECERNAAN SERAT KASAR
| Main Authors: | , Ahmad Wahyudi, Ir.,M.Kes., , Prof. Dr. Ir. Zaenal Bachruddin, M.Sc., |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2012
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/100552/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=56991 |
Daftar Isi:
- In general, crude fiber digestibility in cattle that intensively raise is low due to amount of microbial population and ability are decrease. This study was conducted to obtain superior lignocellulose-degrading bacteria and fungi isolated from buffalo�s and horse�s gastrointestinal tract and elephant�s feces and implement them on in vitro digestibility and on ensilage preparation. The bacteria were isolated anaerobically by Hungate methods, meanwhile fungus were isolated by Rao methods. Lignin/tanic acid, xylan, and cellulose were used as substrates. Lignocellulose degrading activity was determined by qualitative and quantitative analysis. Qualitative analysis was measured based on the ratio of diffusion or clear zone diameter with colony diameter, meanwhile quantitative analysis was done by measured of enzyme activity. The best isolates of bacteria and fungi were determined and implemented for in vitro fiber degestion and ensilage preparation. The result showed that lignocellulose-degrading bacteria and fungi could be isolated from all samples of swamp buffalo and horse digestive�s tract, and also elephant�s feces. The highest number of bacteria and fungi were found from buffalo�s colon as much as 7.5 x 10 8 and 7.2 x 10 6 cfu/g fresh matter respectively. Activity ratio of lignin and xylan-degrading isolates showed very significantly different (P�0. 01), meanwhile cellulose-degrading isolate was significant (P �0. 05). The highest activity ratio of lignin, xylan, and cellulose-degrading bacteria respectively were found from buffalo�s cecum (7.67), horse�s cecum (6.28), and buffalo�s colon (2.39), meanwhile for fungi there were not significant different among isolates from all digestive�s tract compartement. Enzyme activities of lignin and xylan-degrading bacteria showed significanly different (P�0.01) but for cellulose was not different. The superior value of lignin-degrading bacteria were found from elephant�s feces (0.35U/mg protein) and buffalo�s colon (0.24U/mg protein), meanwhile for xylan-degrading bacteria was 33.48 U/mg protein from horse�s cecum. All of enzyme activities of lignin, xylan, and cellulose-degrading fungi showed not significantly different. There were two best species of bacteria based on morphological characterization, i.e. Wautersia paucula (W. paucula) and Enterococcus casseliflavus (E. casseliflavus), and was Aspergillus sp. for fungi. It can be concluded that addition of all bacteria and fungi isolates were able to increase crude fiber (CF), neutral detergent fiber (NDF), and acid detergent fiber (ADF) of rice straw in vitro digestibilities. The most optimal for increasing fiber digestion was reached by E. casseliflavus addition that increased 20.08% CF