IDENTIFIKASI GOLONGAN SENYAWA POTENSIAL PENGHAMBAT PERTUMBUHAN SEL KANKER PAYUDARA (T47D) ORGAN VEGETATIF Dendrobium lasianthera J.J. Sm
| Main Authors: | , Sri Handayani, , Dr. L. Hartanto Nugroho, M.Agr.Sc. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2012
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/100103/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=56633 |
Daftar Isi:
- Dendrobium lasianthera J.J. Sm is a member of genus Dendrobium that have been known having pharmacological effects due to its bioactive compounds. There is no scientific justification about the contents and the benefits of D. lasianthera J.J. Sm bioactive compounds. Therefore, the current research is aimed to identify the potential compound for breast cancer cells (T47D) growth inhibitor from vegetative organs of Dendrobium lasianthera J.J. Sm. D. lasianthera J.J. Sm were collected from Vanindita Nursery, Wedomartani, Ngemplak, Sleman. The materials used were roots, stems and leaves. The materials were macerated using three different solvents, namely chloroform, methanol and water. The extracts were then tested for their toxicity on breast cancer cells (T47D) using -(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay. Potential extracts were fractionated by Vacuum Liquid Chromatography (VLC) method. Potential fraction were isolated using preparative Thin Layer Chromatography (preparative TLC) and the compound was identified by Thin Layer Chromatography (TLC) using silica gel GF254 and sprayed using cerium (IV) sulfate, liebeman-burchard, vanillin sulfate, dragendorff and ammonium, and then was observed under Ultra Violet (UV) light λ 254 and 366 nm. The determination of Inhibitory Concentration of 50% (IC 50) was calculated using linear regression equation. Then IC50 value was test with Analysis Of Variance (ANOVA) and were then test with Duncanâ��s Multiple Rate Test (DMRT) for their significantly different. The results showed that the chloroform extract of D. lasianthera J.J. Sm stem had the lowest IC50 value of 117 Âμg/ml compared to other extracts. The extract were partitioned and tested to obtain the potential cytotoxicity of the fraction. The fraction that have low polarity was the most toxic fraction with IC 50 value of 122.5 Âμg/ml. The low polarity fraction was then separated again. The preparative TLC fraction were tested on cytotoxicity assay in order to obtain the most toxic fraction. The most toxic preparative TLC fraction had IC 50 value of 37.03 Âμg/ml. The IC50 value of the most toxic preparative TLC fraction was lower than the cancer drug, doxorubicine, which had the IC50 value of 61.67 Âμg/ml. The identification of the most toxic preparative TLC fraction showed that the potential growth inhibitor of breast cancer cells (T47D) compounds were terpenoids and phenols.