SYNTHESIS OF Gd-DTPA-FOLAT FOR MAGNETIC RESONANCE IMAGING CONTRAST AGENT AND CHARACTERIZATION BY USING 153Gd-DTPA-FOLATE RADIOACTIVE
| Main Authors: | G., Adang H.; Pusat Radioisotop dan Radiofarmaka (PRR)-BATAN Kawasan Puspiptek Serpong 15314, Tangerang Selatan, S., Yono; Pusat Radioisotop dan Radiofarmaka (PRR)-BATAN Kawasan Puspiptek Serpong 15314, Tangerang Selatan, ., Maskur; Pusat Radioisotop dan Radiofarmaka (PRR)-BATAN Kawasan Puspiptek Serpong 15314, Tangerang Selatan |
|---|---|
| Other Authors: | BATAN |
| Format: | Article application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
BATAN
, 2013
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| Online Access: |
http://jurnal.batan.go.id/index.php/jsmi/article/view/846 |
Daftar Isi:
- Contrast agent was used to clarify the image of the organ that is difficult to distinguish by MRI (Magnetic Resonance Imaging) techniques, particularly in soft tissues of the central nervous system, liver, digestive system, lymphatic system, breast, cardiovascular and pulmonary systems. One of the commonly used contrast agents in hospitals is Gadolinium-DieThylenetriamine Pentaacetic Acid (Gd-DTPA). Gd-DTPA is non specific contrast agent, therefore it has led to develop a contrast agent that is able to achieve the target without defect to the surrounding normal cells. Folic acid as a safe vitamin, inexpensive, specific and also can be used as drug delivery. This study was conducted in the development of targeted MRI contrast agent based of folic acid as a carrier by using Gd metal and DTPA ligand. Since Gd-DTPA-folate is non-active compounds, the characterization of Gd-DTPA-folate was performed using 153Gd-DTPA-folate, which was obtained by reacting DTPA-folate with radionuclides 153Gd. The results of optimization reactions of 153Gd-DTPA-folate was obtained at the mole ratio of DTPA-folate to Gd metal 20:1 and produced the radiochemical purity more than 90%. Identification of the non active product Gd-DTPA-folate was performed using an Fourier Transform- Infra Red (FT-IR) Spectrophotometer and Thin Layer Chromatography (TLC) compared with folic acid and EDA-folate. The results indicated that the product is Gd-DTPA-folate.Keywords: Folic acid, Gd-DTPA, MRI, Contrast agent, 153Gd.