IMMOBILIZATION OF T.REESEI AND S.CELLULOPHYLUM CELLS ON IJUK COVERED WITH HYDROPHILIC AND HYDROPHOBIC POLYMERS BY IRRADIATION TECHNIQUE
| Main Authors: | ., Erizal; Pusat Aplikasi Teknologi Isotop dan Radiasi (PATIR)-BATAN Jl. Cinere Pasar Jum’at, Jakarta 12070, R., Gatot T.; Pusat Aplikasi Teknologi Isotop dan Radiasi (PATIR)-BATAN Jl. Cinere Pasar Jum’at, Jakarta 12070, ., Sudirman; Pusat Teknologi Bahan Iindustri Nuklir (PTBIN)-BATAN Kawasan Puspiptek, Serpong, Tangerang Selatan 15314 |
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| Other Authors: | BATAN |
| Format: | Article application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
BATAN
, 2013
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| Online Access: |
http://jurnal.batan.go.id/index.php/jsmi/article/view/1058 |
Daftar Isi:
- This researchs were done to find out a system available for biomass conversion by immobilization of T.reesei and S.cellulophylum cells on ijuk covered with polymers. Dried ijuks were immersed in the 2-hydroxy ethyl methacrylate (HEMA) and trimethyl propane trimethacrylate (TMPT) solutions at weight ratio of 2/3 and 2/6, then irradiated by using electron beam at a dose of 30 kGy. The matrix covered with the polymers were then immersed in the media containing T.reesei and S.cellulophylum cells at temperature of 28 °C and 48 °C, and incubated at time ranged of 0-350 hours. The enzymes activity produced in the media were tested by reacting with filter paper. The results showed that the GPA (Glucose Paper Activity) values of T.reesei free cells was 2 times higher than immobilized cells on matrix covered with poly (HEMA) and 3 times higher than poly (TMPT). In contrast, the GPA values of immobilized S.cellulophylum cells on matrix covered with poly(HEMA) or poly(TMPT) were ± 2 times higher compared to the free cells.Keywords: Immobilization, Cells, T. Reesei, S. Cellulophylum, TMPT, HEMA.