ANALISIS KUANTITATIF B-KAROTEN DAN UJI AKTTVITAS KAROTENOID DALAM ALGA COKLAT TURBINARIA DECURRENS
| Main Authors: | Biranti, Fransisca; Universitas Diponegoro, Nursid, Muhammad; Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan, Cahyono, Bambang; Organic Chemistry Laboratory, Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Semarang Jalan Prof. Soedarto, Tembalang, Semarang 50275, Telepon +62-24-7474754 |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
JURNAL SAINS DAN MATEMATIKA
, 2010
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| Online Access: |
http://ejournal.undip.ac.id/index.php/sm/article/view/3140 http://ejournal.undip.ac.id/index.php/sm/article/view/3140/2817 |
Daftar Isi:
- ABSTRACT---One of the Indonesian marine natural resources, brown alga of Turbinaria decurrens, used in pharmacy in order of carotenoid pigment as antioxidant. We interested in analysis B-caroten in Turbinaria decurrens and antioxidant and anti-tumor activity. The method that is use to divide carotenoid is the hierarchy maseration method using n-hexane, ethyl acetate and methanol and then using chromatography column. To analyze the qualitative carotenoid uses HPLC by Crc column and eluenmethanol-acetonitrile (3:I). Meanwhile, the test of bioactivity carotene uses radical DPPH (1I'difenil-2-pibilhidrozil) to test of antioxidant qnd sitotoksik asscy with MTT [3,(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrcaolium bromideJ for HeLa tumor cells to test of anti-tumor. The result of this research is B -caroten thqt is in qctract has 0.00387%. Moreover, bioacttvity test shows that B -caroten fraction does notactive to neutralize of DPPHfree radical than ascorbic aci{ but it shows the acttvity to kill HeLa tumor cells.Keywords : Turblnaria decunens, Carolenoid, Antioksidan, Antitumor