Water Fraction Of Sambiloto (Andrographis Paniculata Nees) Ethanol Extract Efficacy In Inducing The Number Of Macrophage, Neutrophil, And The Level Of TNF-α On Wistar Rats
| Main Authors: | Tamayanti, Wahyu Dewi, Kristanti, Lidwina Tri, Kurniawan, Hendra, Ervina, Martha, Hadisoewignyo, Lannie, Soegianto, Lisa, Widharna, Ratna Megawati |
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| Format: | Proceeding PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
, 2013
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| Subjects: | |
| Online Access: |
http://repository.wima.ac.id/6830/1/WATER_FRACTION_OF_SAMBILOTO_%28ANDROGRAPHIS.pdf http://repository.wima.ac.id/6830/ |
Daftar Isi:
- Background. The study of the effect of water fraction of Sambiloto (Andrographis paniculata Nees) ethanol extract on the number of macrophage, neutrophil, and levet of TNF-α in the body fluid of male Wistar rats after Staphylococcus aureus induction. Methods. Animals were divided into 3 groups, namely: negative control (NC), treatment (T), and positive control (PC) group. The NC, T and PC groups were administered by 0,9% NaCL, water fraction of Sambiloto ethanol extract of 40mg/200g BW dose, and ibuprofen of 7.2 mg/200g BW dose, respectively. The treatment was performed for 7 consecutive days. The number of macrophages, neutrophils, was identifed using light microscope whereas the TNF-α. level was identified using ELISA. Outcome Measured. The number of macrophage, neutrophil, and level of TNF-α in the body fluid of male Wistar rats after Staphylococcus aureus induction. Results. The number of macrophages in the KC, the T, and the PC groups were 2.33 ± 1,58; 3.80 ± 4.06; and 2.67 ± 1.55 cells, respectively. The number of neutrophils were 0.83 ± 0.33; 0.58 ± 0.32; and 1.33 ± 0.38 cells respective to the KN, T, and PC groups. The level of TNF-α were I7.78 ± 11.67; 23.48 ± 15.95; and 27.90 ± 30.54 pg/ml respective to the KN, the T, and PC groups, Conclusion. This study indicated that water fraction of Sambiloto ethano! extract able to increase the number of macrophage and TNF-α level yet decrease the number of neutrophil in the body fluids of male Wistar rats after Staphylococcus aureus induction.