The Metformin and hyperbaric oxygen therapy on the Nuclear Factor Kappa B (NF-KB) and Fibroblast Growth Factor (FGF) expression in the wound healing of hyperglycemic rats
| Main Authors: | Dewi, Bernadette Dian Novita, Tamayanti, Wahyu Dewi |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Airlangga University School of Medicine, Surabaya
, 2015
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| Subjects: | |
| Online Access: |
http://repository.wima.ac.id/4198/1/folia%20medica%20indonesiana.pdf http://repository.wima.ac.id/4198/ |
Daftar Isi:
- Complication in diabetes mellitus may occur due to the decline of vasculature resistance and the increased level of oxidative stress matters that lead to the blockage of fibroblast and collagen formation. This, will eventually prolong the wound healing in diabetes mellitus patient, and is recognized as gangrene. This study, therefore, was conducted to elaborate the outcome of metformin and hyperbaric oxygen (HBO) therapy in accelerating the wound healing of hyperglycemic rats which wound was Pseudomonas spp-inoculated by identifying the nucllear factor kappa B (NF-KB) and fibroblast growth factor (FGF) expression. In this study, 50 males Wistar rats were used. The rats were grouped into 5 groups, consisted of 2 control groups and 3 treatment groups. The treatment groups were administered by aloxan, wounded at the right posterior area, and subcutaneously Pseudomonas spp-inoculated. Some groups were administered by metformin or combination of metformin-HBO therapy. Collected data was analyzed Kolmogorov Smirnov to evaluate the significant difference of NF-KB and FGF expression among groups. It was observed that the expression of NF-KB and blood glucose were decreased, however the expression of FGF was increased in the group of rats that treated by the combination of metformin and oxygen hyperbaric (p<0.05). In conclusion, combination of metformin and HBO therapy significantly decreased the expression of NF-KB and increased the FGF expression in the wounded areas of male hiperglycemic rats that Pseudomonas spp inoculated. thus, impicated an acceleration of the wound healing process.