Supercritical CO2Extraction of Phytochemical Compounds from Mimosa pudica Linn
Main Authors: | Rahmawati, Astrid, Pang, Debora, Ju, Yi-Hsu, Soetaredjo, Felycia Edi, Ki, Ong Lu, Ismadji, Suryadi |
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Format: | Article PeerReviewed Book |
Bahasa: | eng |
Terbitan: |
Taylor & Francis Group, LLC
, 2015
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Subjects: | |
Online Access: |
http://repository.wima.ac.id/4177/1/Supercritical%20CO2%20Extraction%20of%20Phytochemical%20Compounds%20%20%20from%20Mimosa%20pudica%20Linn.pdf http://repository.wima.ac.id/4177/ http://dx.doi.org/10.1080/00986445.2014.896346 |
Daftar Isi:
- The extraction of phytochemicals from Mimosa pudica Linn (MPL) using supercritical CO 2 (SC-CO 2 ) has been studied, and the results are presented in this article. The significant variables affecting this extraction were investigated by design of experiment analysis. The effect of temperature and pressure on phytochemical yields was explored by measuring the total phenolic content (TPC), total flavonoid content (TFC), and total alkaloid compound (TAC). The maximum yield of TPC, TFC, and TAC extracted from MPL was 74.83mggallic acid equivalent (GAE) = gdry MPL, 30.93mg quercetin equivalent = gdryMPL, and 14.23mghyos- cyamine (HE) = gdryMPL, respectively. The work of Chrastil was used to correlate the experimental data. It was found that tem- perature and pressure greatly affected the yield of phytochemicals, whereas the effect of extraction time on the extraction yield diminished after 2h. Qualitative analysis of the phytochemicals extracted from MPL was performed by high-performance liquid chromatography (HPLC). The SC-CO 2 extraction was more selective than Soxhlet extraction based on the HPLC spectra.