Effect of Pholiota nameko Polysaccharides Inhibiting Methylglyoxal-Induced Glycation Damage In Vitro
| Main Authors: | Lin, His, Lin, Ting-Yun, Lin, Jer-An, Cheng, Kuan-Chen, Santoso, Shella Permatasari, Chou, Chun-Hsu, Hsieh, Chang-Wei |
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| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
MDPI
, 2021
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| Subjects: | |
| Online Access: |
http://repository.ukwms.ac.id/id/eprint/31471/2/45-Effect_of_pholiota_nameko_.pdf http://repository.ukwms.ac.id/id/eprint/31471/5/45-R1%262-Effect_of_pholiota_nameko_.pdf http://repository.ukwms.ac.id/id/eprint/31471/1/45-Effect%20of%20Pholiota%20nameko_.pdf https://doi.org/10.3390/antiox10101589 http://repository.ukwms.ac.id/id/eprint/31471/ https://www.mdpi.com/journal/antioxidants |
Daftar Isi:
- Advanced glycation end products (AGEs) can induce oxidative stress and inflammation. AGEs are major risk factors for the development of many aging-related diseases, such as cancer and diabetes. In this study, Pholiota nameko polysaccharides (PNPs) were prepared from water extract of P. nameko via graded alcohol precipitation (40%, 60%, and 80% v/v). We explored the in vitro antiglycation ability of the PNPs and inhibition of methylglyoxal (MG)-induced Hs68 cell damage. In a bovine serum albumin (BSA) glycation system, PNPs significantly inhibited the formation of Amadori products. Fluorescence spectrophotometry revealed that the PNPs trapped MG and reduced MG-induced changes in functional groups (carbonyl and ε-NH2) in the BSA. Pretreating Hs68 cells with PNPs enhanced the cell survival rate and protected against MG-induced cell damage. This was due to decreased intracellular ROS content. PNPs thus mitigate skin cell damage and oxidative stress resulting from glycation stress, making them a potential raw material for antiagingrelated skincare products.