Effect of solvent polarity on antioxidant activity during fractionation of ethanolic extract of Citrus hystrix peel

Main Authors: Irawaty, Wenny, Ayucitra, Aning
Format: Proceeding PeerReviewed Book
Bahasa: eng
Terbitan: , 2015
Subjects:
Online Access: http://repository.wima.ac.id/18364/1/11p-Effect_of_solvent_artikel_.pdf
http://repository.wima.ac.id/18364/2/11p-Effect_of_solvent_lengkap_Hasil%20Cek%20Similarity.pdf
http://repository.wima.ac.id/18364/
Daftar Isi:
  • Citrus hystrixhas been claimed to exhibit tremendous performance to repair organ damage caused by free radicals developed during metabolism. The present study was undertaken to evaluate the antioxidant power of Citrus hystrix peel. Antioxidant activity of several fractions (water, ethyl acetate, and hexane) obtained from the fractionation of ethanolic extract of Citrus hystrix peel was investigated. The effect of the solvents on phytochemical content was also evaluated. The research methods involve the Citrus hystrix peel preparation (sizing, drying), extraction of the peel by using ethanol 41% for 8 hours, fractionation of the ethanolic extract by different solvents, and then followed by antioxidant activity measurement of the fractions obtained. The results revealed that all fractions of the ethanolic extract of Citrus hystrix peel exhibited variable antioxidant activity. Specially, the ethyl acetate fraction showed the highest values of antioxidant capacity (% DPPH scavenging activity). The different activity of the fractions was correlated with phytochemical content in each fraction.