Fractionation of phenolic and flavonoid compounds from kaffir lime (Citrus hystrix) peel extract and evaluation of antioxidant activity
| Main Authors: | Wijaya, Yusak Adi, Widyadinata, Daniel, Irawaty, Wenny, Ayucitra, Aning |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Jurusan Teknik Kimia, Univesitas Diponegoro, Semarang
, 2017
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| Subjects: | |
| Online Access: |
http://repository.wima.ac.id/18358/2/8-Fractionation_of_Phenolic_.pdf http://repository.wima.ac.id/18358/1/8-Fractionation_of_Phenolic_Hasil%20Cek%20Similarity.pdf http://repository.wima.ac.id/18358/ https://ejournal.undip.ac.id/index.php/reaktor/article/view/13116 https://doi.org/10.14710/reaktor.17.3.111-117 |
Daftar Isi:
- The observation of side effects during drug consumption results a numerous research to search natural antioxidant such as kaffir lime which has not been utilized. Fruit peel has been reported to exhibit higher antioxidant content than the edible part and therefore, kaffir lime peel was selected in this study. This work was aimed to investigate the effect of solvent polarity (hexane, ethyl acetate and n-butanol) employed during fractionation of ethanolic crude extract and assess its antioxidative activity to neutralize DPPH (2,2-diphenyl-1-picrylhydrazyl) radical. The results show the employment of solvents possessing different polarity resulted fractions of hexane, ethyl acetate and n-butanol with different phenolic and flavonoid amount in each fraction. Semi polar solvent of ethyl acetate performed as the best solvent with total phenolic and flavonoid content was detected 0.1157 mg Gallic Acid Equivalent/mg and 0.1147 mg Rutin Equivalent/mg, respectively. Accordingly, each fraction exhibited different antioxidant activity against DPPH. N-butanol fraction demonstrated the strongest antioxidant activity. Phenolics and flavonoids identification by HPLC (High Performance Liquid Chromatography) indicate the presence different phenolics and flavonoid compounds in each fraction that contribute to antioxidant activity to different extents.