Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica
| Main Authors: | Do, Quy Diem, Angkawijaya, Artik Elisa, Tran-Nguyen, Phuong Lan, Huynh, Lien Huong, Soetaredjo, Felycia Edi, Ismadji, Suryadi, Ju, Yi-Hsu |
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| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Elsevier
, 2014
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| Subjects: | |
| Online Access: |
http://repository.wima.ac.id/11256/1/1-s2.0-S1021949813001348-main.pdf http://repository.wima.ac.id/11256/12/26-R%201%20%26%202%20Effect%20of%20extraction%20solvent%20..........pdf http://repository.wima.ac.id/11256/ http://www.jfda-online.com/article/S1021-9498(13)00134-8/abstract |
Daftar Isi:
- Limnophila aromatic is commonly used as a spice and a medicinal herb in Southeast Asia. In this study, water and various concentrations (50%, 75%, and 100%) of methanol, ethanol, and acetone in water were used as solvent in the extraction of L. aromatica. The antioxidant activity, total phenolic content, and total flavonoid content of the freeze-dried L. aromatic extracts were investigated using various in vitro assays. The extract obtained by 100% ethanol showed the highest total antioxidant activity, reducing power and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity. The same extract also exhibited the highest phenolic content (40.5 mg gallic acid equivalent/g of defatted L. aromatic) and the highest flavonoid content (31.11 mg quercetin equivalent/g of defatted L. aromatic). The highest extraction yield was obtained by using 50% aqueous acetone. These results indicate that L. aromatica can be used in dietary applications with a potential to reduce oxidative stress.