PROTEIN PROFILE ANALYSIS ON LOCAL BEAN(KORO) USING SDS_PAGE

Main Authors: Retnaningsih, Christiana, Pratiwi, Alberta Rika
Format: Article PeerReviewed Book
Bahasa: eng
Terbitan: , 2018
Subjects:
Online Access: http://repository.unika.ac.id/23761/1/58119951852017G2_ST%26%20Materi%20P3FNI_2.pdf
http://repository.unika.ac.id/23761/
Daftar Isi:
  • Koro is one type of local beans that could be found widespread in Indonesia. There are several types of Koro with specific characteristics such as the shape, colour and active compounds. Koro is high in protein but low in fat. There are three species of Koro widely planted in Central of Java such as Mucuna pruriens, L (Velvet bean or Koro Benguk), Canavalia ensiformis, L. (jack bean or Koro Pedang), and Phaseolus lunatus, L. (Lima bean or Koro Glinding). This study aimed to determine the protein profile of three species of Koro. Protein content was analyzed using Kjeldal method, protein molecular weight using SDS-PAGE, and identification of protein with bioinformatics method. The result of protein content showed that velvet beans have the highest isolate protein content of 97,713%. The isolate protein content of jack beans was 70,897% and the isolate protein content of lima bean was 63%. Jack beans had 5 types of protein with molecular weight respectively 210 kDa. Lima beans had 8 types of protein with molecular weight respectively 210 kDa, 104.2 kDa,68 kDa, 56.2 kDa, 39,4 kDa, 34 kDa, 22 kDa. Velvet beans had 7 typs of protein with molecular weight resprctively 195 kDa, 104.2 KDa, 56.2 kDa, 34 kDa, 25.1 kDa, 22 kDa, 10.4 kDa. Lima beans had 8 types of protein with molecular weight respectively 210 kDa, 104.2 kDa, 56.2 kDa, 34 kDa, 29 kDa, 25.1 kDa, 22 kDa, 10.4 kDa. The result of bioinformatics analysis were protein fosfatase, levodopa, albumine, canavaline, legumine, concanavalin A, oleosin, lectin, chitinase, chymotripsin inhibitor, and calmodulin.