UJI AKTIVITAS SITOTOKSIK EKSTRAK KULIT JERUK PURUT (Citrus hystrix) PADA SEL HeLa CERVICAL CANCER CELL LINE
| Main Author: | Nathanael, Joshua |
|---|---|
| Format: | Thesis NonPeerReviewed Book |
| Terbitan: |
, 2015
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| Subjects: | |
| Online Access: |
http://e-journal.uajy.ac.id/6974/1/BL001171.pdf http://e-journal.uajy.ac.id/6974/2/BL101171.pdf http://e-journal.uajy.ac.id/6974/3/BL201171.pdf http://e-journal.uajy.ac.id/6974/4/BL301171.pdf http://e-journal.uajy.ac.id/6974/5/BL401171.pdf http://e-journal.uajy.ac.id/6974/6/BL501171.pdf http://e-journal.uajy.ac.id/6974/ |
Daftar Isi:
- Citrus hystrix or know as kefir lime in Indonesia were not used as medicine. Kefir lime were more used as skin and body treatment rather as food composition. The research of cytotoxic effect on HeLa from ethanolic extract of kefir lime peels is never been done before, ethanolic extract of kefir lime peels were used on this research. The point of this research is to know the main compound that built ethanolic extract of kefir lime peels and to know the cytotoxic effect of ethanolic extract from kefir lime peels. Thin layer chromatography (TLC) were being used to identify the secondary metabolites in citrus peels extract. From the TLC Alkaloids, Flavonoids, Terpenoids, Tanin, and Saponin, were identified. The method that being used to identify the cytotoxic effect of citrus peel extract in this research were MTT assay. From the MTT assay we can obtain the IC50 of the sample. The IC50 of this Kefir lime peel extract is 873,277 μg/mL.