TLC-Fingerprinting and Chemometrics for Identification of Curcuma xanthorrhiza From Different Geographical Origins
| Main Authors: | Jayani, Nikmatul Ikhrom Eka, Haridsa, Nindya Maulita, Sabatini, Syahna Selinda, Setiawan, Finna, Hadiyat, Mochammad Arbi, Kartini, Kartini |
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| Format: | Lainnya NonPeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
fakultas farmasi universitas surabaya
, 2022
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| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/42388/ |
Daftar Isi:
- Geographical origin was an important parameter that influences the quality of herbal medicine. The fingerprint of herbal medicine would be described as the chemical compound related to the quality. Curcuma xanthorrhiza (Zingiberaceae) which popular as Java Turmeric was widely used in Indonesian traditional medicine. This study was to validated and developed a TLC (Thin Layer Chromatography) fingerprint of Java Turmeric. Chemometric techniques were done to classified the quality of Java Turmeric from 15 regions in Indonesia. The methods refer to TLC fingerprint, validity test (stability and precision), and chemometric analysis with PCA (Principal Component Analysis) and CA (Clustering analysis). TLC-fingerprints of Java Turmeric resulted in 5 zones under chromatographic conditions (TLC plate of Silica gel F254, mobile phase: DCM, CHCL3, and EtOH (10: 10: 1), detection with vanillin-sulfuric acid reagent). Chromatogram was stable both on the TLC plate and in the solution, stable during the chromatography process (2D chromatogram), and stable within 60 minutes (after derivatization). The chromatogram meets intraday precision, on the other hand, does not meet inter-day precision. Chemometric analysis with PCA and CA showed the sample were divided into 5 clusters, namely cluster 1 (Tawangmangu, Bangkalan, Kediri and Surabaya); Cluster 2 (Batu, Sragen, Tulungagung, Pasuruan, Blitar, and Central Lombok); Cluster 3 (Ngawi); Group 4 (Gresik); and Cluster 5 (Bojonegoro, Banyuwangi, and Palangkaraya). Conclusion: Java Turmerics from 15 regions in Indonesia were divided into 5 clusters based on the similarity of the chemical compounds. The TLC fingerprint-chemometric methods on Java Turmeric were useful for quality control based on geographic origin and identified the authenticity.