Callus-mediated Somatic Embryogenesis and Plant Regeneration in Vanda tricolor Lindl.var.Pallida
| Main Authors: | Hardjo, Popy Hartatie, Savitri, Wina Dian, Artadana, Ida Bagus Made, Putra, Sulistyo Emantoko Dwi |
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| Format: | Article PeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
Hashemite University
, 2021
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| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/40920/ https://jjbs.hu.edu.jo/files/vol14/n5/Paper%20Number%208.pdf |
Daftar Isi:
- In this study, a protocol to induce indirect somatic embryogenesis from the basal leaf segments of Vanda tricolor Lindl. var. Pallida has been developed. 2 0 T The experiments consisted of two stages، i.e. induction of SEs from calli and regeneration of plantlet from SEs. 2 0 T Embryogenic calli obtained from previous experiment (0.05 mg LP –1P NAA + 0.01 mg LP –1P BAP) were used to induce somatic embryos (SEs) on half-strength Murashige and Skoog (MS) medium supplemented with (0.05 mg L P –1 P toP P 0.20 mg LP –1P ) 6 -benzylaminopurine (BAP) alone or in combination with 0.01 mg L P –1 P α-naphtalene acetic acid P P (NAA). Embryogenic calli, those cultured on 0.05 mg LP –1P BAP and 0.01 mg LP –1 P NAA resulted in 90 % induction of SE structures at 30 d of culture period. Histological observation exhibited development of pro-embryo to form completed embryo. The pattern of SEs development started from embryogenic callus to form pro-embryo, followed by globular phase at 10 d of culture. Globular embryo elongated to form suspensor at 20 d of incubation period, and completed embryo. Regeneration of SEs into compl ete plantlets was attained on the half -strength MS medium without addition of any plant growth regulator (PGR). Based on the results of the present study, it can be concluded that 2 0 T half-strength MS medium supplemented with 2 0 T NAA 0.01 mg LP –1P R Rand BAP 0.05 mg LP –1P is the best medium for induction of SEs from embryogenic calli.