Thin Layer Chromatography Fingerprinting and Clustering of Orthosiphon stamineus Benth. from Different Origins
| Main Authors: | Kartini, Kartini, Jayani, Nikmatul Ikhrom Eka, Hadiyat, M. Arbi , Avanti, Christina |
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| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
Pharmacognosy Network Worldwide
, 2020
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| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/37251/1/PJ%2D12%2D1%2D304_0.pdf http://www.phcogj.com/article/1088 http://repository.ubaya.ac.id/37251/ |
Daftar Isi:
- Introduction: Orthosiphon stamineus has been widely used across Asian countries for the treatment of various diseases. The quality of herbal medicine determine its safety as well as efficacy; and geographical origin is important factor contributing on the quality of herb and its products. Materials and Methods: Thin Layer Chromatography (TLC) method combined with chemometric, Principal Component Analysis (PCA), has been employed to evaluate the quality of Orthosiphon stamineus leaves collected from eleven origins in Indonesia. Results: The results showed that mobile phase suitable for Orthosiphon stamineus was chloroform, dichloromethane, ethyl acetate (7:4:1). The method used has met the requirements of TLC system stability and precision. TLC-fingerprints analyzed with chemometrics showed an ability to discriminate Orthosiphon stamineus from various origins. PCA score plot of the first two principal components (PC) clearly distinguished 3 clusters of samples, whereas the loading plot of the first two PC showed that compounds with the Rf values of 0.0-0.1, 0.1-0.2, 0.2-0.3, and 0.9-1.0 are the most important compounds for clustering of samples. Conclusions: TLCfingerprint combined with the PCA was able to discriminate among the leaves of Orthosiphon stamineus originated from various locations. TLC-fingerprints analyzed with chemometrics can be used as an alternative of marker-oriented method to evaluate the quality of Orthosiphon stamineus. Key words: Geographical origin; Herbal medicine; Marker; Principal component analysis; Quality; TLC.