Simultaneous HPLC determination of Lidocaine Hydrochloride and Hexachlorophene in a Suppository Product
| Main Author: | Furwanti, Cece |
|---|---|
| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
Fakultas Farmasi Universitas Surabaya
, 2020
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| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/37017/1/Cece_Simultaneous%20HPLC%20determination_Abstrak.pdf http://repository.ubaya.ac.id/37017/2/Cece_Simultaneous%20HPLC%20determination.pdf http://repository.ubaya.ac.id/37017/ |
Daftar Isi:
- Introduction:Antihemoroid® suppository has been produced commercially by PT Kimia Farma, Indonesia. For QC purposes, a separated densitometric method for analysis of its active ingredients, lidocaine hydrochloride and hexachlorophene, was applied. Objective:The objective of this study was obtaining more efficient analysis method of LH and HC, therefore an HPLC procedure has been developed for the determination of both compounds simultaneously. Methods:AYMC-Triart C18 column was used with a gradient mobile phase consisting of acetonitrile and phosphate buffer 0.05 M (pH 6.0). Quantitative evaluation was performed at 220 nm. Method validation was performed according to the new methods of USP 41. Result: Showed that the HPLC method was simple, accurate, precise, and robust. Conclusion:The HPLC method can be applied in simultaneous determination of LH and HC in suppositories as a QC tool in the pharmaceutical industries.