PRELIMINARY STUDY ON ANTIOXIDANT PROPERTY OF TYPHONIUM FLAGELLIFORME AND ITS INTERACTION WITH DNA
| Main Authors: | Purwanto, Maria Goretti Marianti, Tjie , Kok, Melinda, Ratna |
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| Format: | BookSection PeerReviewed application/pdf |
| Terbitan: |
Universitas Ma Chung
, 2011
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| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/3498/1/Preliminary%20study_Abstract_2011.pdf http://repository.ubaya.ac.id/3498/2/Poster_preliminary%20study_2011.pdf http://repository.ubaya.ac.id/3498/ |
Daftar Isi:
- The research was conducted in order to detect active chemicals of Typhoniumflagelliforme with antioxidant property and at the same time it is also able to interact with DNA. The activity of each extract was determined by evaluation of antioxidant activity using DPPH reagent. The most active extract of Typhoniumflagelliforme was further purified by a column chromatography. The result showed that the highest antioxidant activity was given by dichloromethane extract of the root with IC50= 245.989 ppm. The capability of interaction with DNA was investigated by Thermal Denaturation Assay. Changes in DNA melting temperature (Tm) indicated the interaction between DNA and the constituents of the most active fraction. There are more than one active constituent indicated by TLC analysis. TLC spots were visualized at 365 nm and sprayed by visualization reagent to detect alkaloids, terpenoids and flavonoids qualitatively. Phytochemistry analysis showed that the most active fraction of Typhoniumflagelliforme possibly contains alkaloids, terpenoids and flavonoids.