HPTLC Fingerprints of Some Indonesian Medicinal Plants Used in “Scientific Jamu”
| Main Authors: | Kartini, Kartini, Ekajayani, N.I, Oktaviyanti, Nina Dewi |
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| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/34902/12/Kartini_HPTLC%20Asia%202018_Book%20of%20Abstracts%20_Final_2018.pdf http://repository.ubaya.ac.id/34902/ |
Daftar Isi:
- The use of herbal medicine in Indonesia is still very popular. However, it has not been recognized by the medical doctors as an alternative method of health care. Therefore, the government created a “Scientific Jamu” program, which is a scientific proof of herbal medicine through health care-based research. To succeed this program, supports from various aspects is needed, including ensuring the authenticity and quality of the raw materials. In this research, the HPTLC fingerprint of seven medicinal plants (i.e.: Orthosiphon stamineus, Centella asiatica, Phyllanthus niruri, Apium graveolens, Caesalpinia sappan, Stelechocarpus burahol, and Curcuma domestica) used in “Scientific Jamu” were established. Optimization of the mobile phase was designed with the mixture design method to generate separation with the highest number of bands and the best separation. Validation methods included stability, precision (intraday and interday), and specificity. Each plant was sonicated using methanol and then proceeded for HPTLC analysis using the following condition. A Camag TLC system comprising of Linomat 5 sample applicator, twin-through chamber, TLC visualizer 2, and Visioncats software were used. Chromatography was performed on HPTLC plates (silica gel 60 F254) with a 100-l Camag syringe. Samples were spotted under a flow of N2 as 6 mm bands, 15 mm from the left edge, 10 mm from the bottom edge and 10 mm of track distance. Development (80 mm) was carried out in a chamber previously equilibrated with mobile phase which were optimized for each plant extract. The plates were dried under warm air and derivatized using a specific reagent. Fingerprints evaluation was carried out under TLC visualizer. The fingerprints of the seven plants is presented at Fig.1. Method validation exhibited that the method used meets the requirements of stability, precision and specificity. It can be concluded that developed HPTLC fingerprint can be used to ensure the authenticity of plants used in “Scientific Jamu”.