Reduction of a-tocopherylquinone Model Compound With Various Reductant
| Main Author: | Kohar, Indrajati |
|---|---|
| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
University of Surabaya
, 2001
|
| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/33/1/Art0001_Indrajati.pdf http://repository.ubaya.ac.id/33/ |
Daftar Isi:
- In order to study the possibility of tranformation of a-tocopherylquinone (TQ) into a more oxidiseable compound and also to find out the recycling effect in the cells, an experiment was conducted by reducing the model compound 2-(3- hydroxy-3-methylbutyl)-3,5,6-trimethyl-1,4-benzoquinone (PQ) with various reductants. In the experiment it was shown that glutathione did not reduce PQ,nor NADH by itself, so the effective reductant in the NADH/FAD combination must have been FADH2. Thus there is a probability that in a biological system, the most probable reductant for TQ would be a flavin enzyme rather that ascorbic acid or glutathione. The non-physiological dithiothreitol was as effective as NADH/ FAD which is interesting because of its similarity to the physiologically important reduced lipoic acid. The reactivity of the various reductants used in this experiment decrease in the order of dithiothreitol ~ NADH/FAD (8/10) > sodium dithionite > NADH/FAD (2:10) > sodium ascorbate > ascorbic acid (Fig.8).