Optimization of DNA extraction from seeds and fresh leaf tissues of Soybean (Glycine max)
| Main Authors: | Yunita, Oeke , Setiawan, Benny , ., Evellyn |
|---|---|
| Format: | Proceeding PeerReviewed |
| Terbitan: |
, 2012
|
| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/31725/ |
Daftar Isi:
- The effects of various components on extraction buffer such as SDS-NaCI, PVP, p-mercaptoethanol, extraction with Phenol:Chloroform:lsoamyl acetate, and incubation time on the DNA extraction from seeds and fresh leaves of Soybean (Glycine max)were studied. Based on results above, an optimized method for DNA extraction from Soybean seeds and leaves were established. Extracting Soybean seeds twice with Phenol:Chloroform:lsoamyl acetate (25:24:L), incubating for 30 min and 7% SDS-Z M NaCl in extraction solution could promote the quantity and purity of DNA from seeds, respectively. The results also showed that high quality DNA from Soybean leaves could be extracted with Phenol:Chloroform:lsoamyl acetate (25:24:t) twice, incubating for 30 min and A5% SDS-2% PVP in extraction solution, although the DNA quantity was less than DNA quantity obtained with Nucleospin' Plant ll method.