Functional characterization of the pathogenesis-related protein family 10 gene, PgPR10-4, from Panax ginseng in response to environmental stresses
| Main Authors: | Kim, Yu-Jin, Jang, Moon-Gi , Lee, Hye-Jin , Jang, Gyu-Hwan , Sukweenadhi, Johan, Kwon, Woo-Saeng , Yang, Deok-Chun |
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| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
Springer Netherlands
, 2014
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| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/31174/7/Kim_Abstract_2014.pdf http://repository.ubaya.ac.id/31174/1/kim2014.pdf https://link.springer.com/article/10.1007/s11240-014-0505-5 http://repository.ubaya.ac.id/31174/ |
Daftar Isi:
- Pathogenesis-related proteins (PRs) are known to function in higher plants as a protein-based defensive system against abiotic and biotic stress, particularly pathogen infections. A full-length cDNA sequence of PR BetV1 was isolated and characterized from a 14-year-old ginseng expressed sequence tags library and we named this as PgPR10-4, because of similar identities with previous isolated PgPR10s sequences. The PgPR10-4 gene encodes a 477 bp open reading frame and its deduced protein contains 158 amino acids with a 53 % identity with that of the Actinidia chinensis BetV1 allergen. The expression of PgPR10-4 gene was abundant in leaves and its transcripts showed differentially up-regulated patterns against several ginseng pathogens and abiotic stimuli such as high light and salinity. In addition, PgPR10-4 expression was strongly responsive towards the stress signaling molecules H2O2 and jasmonic acid (JA), while weekly responsive to salicylic acid and abscisic acid. A functional role of PgPR10-4 in environmental stress tolerance was further validated through its overexpression in Arabidopsis. An analysis of T2 transgenic Arabidopsis plants overexpressing the PgPR10-4 gene showed an enhanced tolerance to bacterial and fungal infection, but not to salt stress. When we tagged with cyan fluorescent protein fusion protein, the PgPR10-4-was found to localize to the cytoplasm. The enhanced antifungal activity observed from the Arabidopsis transgenic lines suggests the possible involvement of PgPR10-4 in a defense-related mechanism via the JA signaling pathway.