Immobilization of Chondroitin Sulfate A into Monolithic Epoxy Column for Chiral Separation
| Main Authors: | ., Ratih, Asmari, Mufarreh , Öner, Selin , El Deeb, Sami |
|---|---|
| Format: | Proceeding PeerReviewed application/pdf |
| Terbitan: |
, 2017
|
| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/30262/1/Immobilization%20of%20Chondroitin%20Sulfate%20A.pdf https://goo.gl/kYTxrD http://repository.ubaya.ac.id/30262/ |
Daftar Isi:
- Chondroitin sulfate A was successfully immobilized into epoxy monolithic column at a concentration of 3% (w/v) in the presence of ethylene diamine. The epoxy group of monolithic column was first converted to aldehyde group by successive hydrolization and oxidation. A Schiff base reaction at pH 8.0 was used to attach the diamine-spacer to aldehyde group. The chondroitin sulfate A was introduced into the monolithic column by circulating the solution at a flow rate of 0.1 mL/min for 24 hours. The chondroitin sulfate A-immobilized epoxy column was evaluated for chiral separation of verapamil enantiomers under optimized HPLC conditions at a wavelength of 230 nm. As a mobile phase, 20 mM Na2HPO4 (pH 2.9) was used. A resolution (Rs) of about 1.5 was achieved for the separation of verapamil enantiomers. A good repeatability of the retention time at two concentration levels (n=8) with RSD < 1% was obtained. The linear responses of verapamil enantiomers were in the range of 1.0-3.0 ppm with R2 of about 0.994.