ISOLATION AND CHARACTERIZATION OF ENDOPHYTIC BACTERIA FROM THE LEAF EXPLANTS OF Avicennia marina (Forsk.)
| Main Authors: | Savitri, Wina Dian, Wirjaputra, Maria Veronica , Hardjo, Popy Hartatie |
|---|---|
| Format: | Proceeding NonPeerReviewed application/pdf |
| Terbitan: |
, 2016
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| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/28755/7/Isolation%20and%20Characterization_2016.pdf http://repository.ubaya.ac.id/28755/ |
Daftar Isi:
- Mangroves have important role in maintaining coastal area ecosystem as well as in protecting it from abrasion. The overexploitation of mangroves is of today's concern because it threatened the sustainability of the ecosystems. Avicennia marina (Forsk.) is one of mangroves that grow in Ekowisata Mangrove Wonorejo Surabaya. The previous aim of the experiment was to clone this species by plant tissue culture method in order to provide a sufficient number of seedlings for reforestation. However, there were a lot of obstacles in obtaining the sterile explants because the contaminants that live inside the leaf samples (endophytic). The isolation of endophytic bacteria was done after the surface sterilized leaf discs were cultured on MS and PCA media. The characterization was conducted biochemically based on Bergey's manual of Determinative Bacteriology. The results confidently showed that there were two genera of endophytic bacteria inside the leaves of Avicennia marina (Forsk.), i.e. Bacillus and Corynebacterium. However, there was also another genus but still not sure yet whether it was Shigella or Yersinia. The results were beneficial to further investigate the strategy to be able to obtain sterile leaf explants of Avicennia marina (Forsk.)