Construction of Bacillus subtilis Spizizenii W23 Mutant Transposon for Biohydrogen Production
| Main Authors: | Wahjudi, Mariana , Sidarta, Michelle Margaret, Liasari, Yusnita, Daniel, Xavier |
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| Format: | BookSection PeerReviewed application/pdf |
| Terbitan: |
Universitas Islam Sumatra Utara (UISU)
, 2014
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| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/24596/1/Construction%20of%20Bacillus%20subtilis_Abstract_2014.pdf http://repository.ubaya.ac.id/24596/2/Construction%20of%20Bacillus%20subtilis_2014.pdf http://repository.ubaya.ac.id/24596/ |
Daftar Isi:
- Several bacteria, such as Bacillus sp. were reported can produce biohydrogen, the environmentally-friendly-renewable energy. However, not all of the species belong to the genus Bacillus is a bio-hydrogen producers. B. subtilis is one of the non-hydrogen-producer bacilli although there are several genes on its genomic sequence that are predicted involved in the pathways. In this study, we improve the ability of B. subtilisspizizenii W23 to produce biohydrogen through transposon mutagenesis. The B. subtilis W23 strain mutagenesis was performed through genomic insertion with a Tn5-transposon derived from pUTmini-Tn5-luxCDABE-Km plasmid Transformantswere screened on Luria-Bertani plates containing kanamycin 30ug/ml and then were selected based on the loweringacid production, usingpHindicatorphenolredon the medium, spectropbotometrically and acid probe based assays. From 133 transformants grown on LB-kanamycin plates, 131 of them showed decreased in its acid productions. There were two mutants; number99andl26 that producedbiohydrogen inmineral-glucosemedium. Transposon positions of both mutants were determined