The Role and Efficiency of Ammonium Sulphate Precipitation in Purification Process of Papain Crude Extract
| Main Author: | Purwanto, Maria Goretti Marianti |
|---|---|
| Format: | Proceeding PeerReviewed application/pdf |
| Terbitan: |
, 2015
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| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/24504/1/Purwanto_The%20Role%20and%20Efficiency_Abstract_2015.pdf http://repository.ubaya.ac.id/24504/6/Maria%20Goretti_The%20Role%20and%20Efficiency%20of%20Ammonium....pdf http://repository.ubaya.ac.id/24504/ |
Daftar Isi:
- It has been common to do fractionation (for example using ammonium sulphate as a precipitating agent) before doing a more sophisticated method for purification of protein. The logic background is easy to understand, but in fact, the precipitation step often caused severe lost in yield and activity of the protein, making the whole purification effort too costly. In this work we evaluate the specific activity (thus, purification factor) and total activity (yield) during the purification process of papain crude extract using Ion Exchange Chromatography . (IEC), with and without prior fractionation using ammonium sulphate. Detail phenomenon in each step was also recorded, Including the UV spectra and SDS-PAGE profile of the intermediate purification products.