Quorum-Quenching Acylase Reduces the Virulence of Pseudomonas aeruginosa in a Caenorhabditis elegans Infection Model
| Main Authors: | Papaioannou, Evelina, Wahjudi, Mariana , Jimenez, Pol Nadal, Koch, Gudrun, Setroikromo, Rita, Quax, Wim J. |
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| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
American Societyfor Microbiology
, 2009
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| Subjects: | |
| Online Access: |
http://repository.ubaya.ac.id/20920/1/Quorum%2DQuenching_Abstract_2009.pdf http://repository.ubaya.ac.id/20920/7/Quorum%2DQuenching_2009.pdf http://aac.asm.org/content/53/11/4891.full http://repository.ubaya.ac.id/20920/ |
Daftar Isi:
- The Pseudomonas aeruginosa PAO1 gene pvdQ encodes an acyl-homoserine lactone (AHL) acylase capable of degrading N-(3-oxododecanoyl)-l-homoserine lactone by cleaving the AHL amide. PvdQ has been proven to function as a quorum quencher in vitro in a number of phenotypic assays. To address the question of whether PvdQ also shows quorum-quenching properties in vivo, an infection model based on the nematode Caenorhabditis elegans was explored. In a fast-acting paralysis assay, strain PAO1(pMEpvdQ), which overproduces PvdQ, was shown to be less virulent than the wild-type strain. More than 75% of the nematodes exposed to PAO1(pMEpvdQ) survived and continued to grow when using this strain as a food source. Interestingly, in a slow-killing assay monitoring the survival of the nematodes throughout a 4-day course, strain PAO1-ΔpvdQ was shown to be more virulent than the wild-type strain, confirming the role of PvdQ as a virulence-reducing agent. It was observed that larval stage 1 (L1) to L3-stage larvae benefit much more from protection by PvdQ than L4 worms. Finally, purified PvdQ protein was added to C. elegans worms infected with wild-type PAO1, and this resulted in reduced pathogenicity and increased the life span of the nematodes. From our observations we can conclude that PvdQ might be a strong candidate for antibacterial therapy against Pseudomonas infections.