THE EFFECT OF ANTIFUNGAL ACTIVITY OF β-1,3-GLUCANASE Trichoderma reesei AGAINST ROOT FUNGI OF Ganoderma philippii
| Main Authors: | Budiarti, Sri Wahyuni; Balai Pengkajian Teknologi Pertanian (BPTP) Jawa Tengah Bukit Tegalepek Ungaran Jawa Tengah 50501, Widyastuti, S.M.; Fakultas Kehutanan, Universitas Gadjah Mada, Jalan Agro No 01 Bulaksumur Yogyakarta 55281 |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Pusbindiklat Peneliti - LIPI
, 2011
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| Subjects: | |
| Online Access: |
http://widyariset.pusbindiklat.lipi.go.id/index.php/widyariset/article/view/435 http://widyariset.pusbindiklat.lipi.go.id/index.php/widyariset/article/view/435/357 |
Daftar Isi:
- Purifi ed three isoform β-1,3-glucanases of 90 kDa β-1,3-glucanase-I, 75 kDa β-1,3-glucanase-II, and 64 kDa β-1,3-glucanase-III derived from Trichoderma reesei isolate T13were tested in vitro for its antifungal activity to the root fungi of Ganoderma philippii. Antifungal activity was tested by bioassay using a modifi ed cup plate method and microscopic observation to determine the presence of abnormal mycelia growth of G. philippii. The result showed that antifungal activity of those three isoform β-1 ,3-glucanase (β-1,3-glucanase-I, β-1,3 glucanase-II, and β-1,3-glucanase-III) in vitro could not form growth inhibition against the mycelia of G. philippii. Microscopic observation revealed that the enzyme caused necrotic lesion, whereas the combination of β-1,3-glucanase and chitinase from T. harzianum (Sigma) caused lyzed hyphae of G. philippii.