The Effect of Red Dragon Fruit (Hylocereus polyrhizus) Rind Ethanol Extract on Collagen Deposition in The Liver of Menopause Model Rats
| Main Authors: | Kenjiro, Eduardo, Arijana, I Gusti Kamasan Nyoman, Sugiritama, I Wayan, Ratnayanti, I Gusti Ayu Dewi |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Universitas Udayana
, 2023
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| Online Access: |
https://ojs.unud.ac.id/index.php/eum/article/view/95895 https://ojs.unud.ac.id/index.php/eum/article/view/95895/50715 |
Daftar Isi:
- Background : Estrogen is a steroid hormone that is not only the primary sexual hormone in women, but also functions to protect the body from many damaging conditions, especially in the liver. Red dragon fruit (Hylocereus polyrhizus) rind is known to have a higher antioxidant potential than the fruit flesh and has the potential to be an alternative solution for this condition. Objective : To determine the effect of giving Hylocerus polyrhizus rind extract on collagen deposition in the livers of menopausal wistar rats. Methods : This randomized post-test only control group design study was conducted on in vivo experimental animals in the form of rats that had undergone the ovariectomy procedure and were randomly divided into three groups. Group P0 served as the control without intervention, P1 with the intervention of ethanol extract of red dragon fruit rind at a dose of 60 body weight/200gr/day, while P2 with the intervention at a dose of 90 body weight/200grBB/day. Results : Of the 30 mice divided into three groups, the mean area of collagen deposition was obtained in the P0 (24,130 ± 7,205), P1 (18,325 ± 4,221) and P2 groups (12,576 ± 3,739). The test results with One Way ANOVA found a significant difference with a value of p = 0.000189. The results of the LSD Post Hoc test found a significant difference in mean between the P0 and P2 groups (p = 0.00017). Conclusion : Administration of Hylocereus polyrhizus ethanol extract inhibited the expansion of collagen deposition in the treatment group's livers when compared to the control group's livers.
- Latar belakang : Estrogen adalah hormon steroid yang tak hanya menjadi hormon primer seksual pada Wanita, tetapi juga berfungsi melindungi tubuh dari begitu banyak kondisi kerusakan, utamanya pada hepar. Kulit buah naga merah (Hylocereus polyrhizus) diketahui memiliki potensi antioksidan yang lebih tinggi daripada daging buahnya dan berpeluang menjadi solusi alternatif bagi kondisi ini. Tujuan : Mengetahui pengaruh pemberian ekstrak kulit Hylocerus polyrhizus terhadap deposisi kolagen pada hepar tikus wistar model menopause. Metode : Penelitian randomized post-test only control group design ini dilakukan pada hewan coba in vivo berupa tikus yang sudah melalui prosedur ovariektomi dan secara acak dibagi ke dalam tiga kelompok. Kelompok P0 sebagai kontrol tanpa intervensi, P1 dengan intervensi ekstrak etanol kulit buah naga merah dosis 60mg/200grBB/hari, dan P2 dengan intervensi ekstrak etanol kulit buah naga merah dosis 90mg/200grBB/hari. Hasil : Dari 30 tikus yang dibagi menjadi tiga kelompok, didapatkan rerata luas deposisi kolagen tikus model menopause pada kelompok P0 (24,130 ± 7,205), P1 (18,325 ± 4,221), dan P2 (12,576 ± 3,739). Hasil uji dengan One Way ANOVA ditemukan adanya perbedaan yang bermakna dengan nilai p = 0,000189. Hasil uji Post Hoc LSD ditemukan perbedaan rerata yang bermakna antara kelompok P0 dengan P2 (p = 0,00017). Simpulan : Pemberian ekstrak etanol kulit buah Hylocereus polyrhizus menghambat perluasan deposisi kolagen pada hepar kelompok perlakuan jika dibandingkan dengan hepar kelompok kontrol.