Inhibition Test of Sambiloto (Andrographis paniculata Nees) and Moringa (Moringa oleifera L.) Leaves Ethanol Extract Against Streptococcus pyogenes Bacteria
| Main Authors: | Pratiwi, Kadek Prastiti Surya, Jawi, I Made, Indrayani, Agung Wiwiek, Mahendra, Agung Nova |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Universitas Udayana
, 2023
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| Online Access: |
https://ojs.unud.ac.id/index.php/eum/article/view/81175 https://ojs.unud.ac.id/index.php/eum/article/view/81175/51752 |
Daftar Isi:
- Cases of infection and antibiotic resistance by Streptococcus pyogenes are increasing. Sambiloto leaf has unique phytochemical, namely andrographolide as antibacterial and other phytochemicals such as alkaloids, flavonoids, phenols, saponins. Meanwhile, moringa leaves which are often consumed as vegetables have antibacterial activity with phytochemicals including vitamin C, alkaloids, flavonoids, phenolics, triterpenoids, and tannins. This study aimed to analyze the inhibition of the ethanolic extract of sambiloto leaf, moringa leaf, and their combination on the growth of Streptococcus pyogenes bacteria in vitro. This type of research is a research using true experimental post-test only control group design. The sample was divided into 2 groups: control group and the treatment group with variations in concentrations of 25%, 50%, 75%, and 100%. Data were analyzed using the SPSS application using Kruskal-Wallis method with p<0.05. The results showed that the ethanolic extract of sambiloto leaves had no inhibition against Streptococcus pyogenes at a concentration of 25%, but had the highest inhibitory power at a concentration of 100%, which was 8.67 mm. The highest inhibitory power of moringa leaf ethanol extract was at 100%, which was 9 mm. The inhibition zone produced by the combination of the ethanol extract of sambiloto and moringa leaves showed a synergistic effect at a concentration of 100%, which was 9.33 mm. The positive control used amoxicillin with an inhibitory zone of 26.4 mm. Sambiloto leaf extract, moringa leaf, and their combination have an inhibitory effect on the growth of Streptococcus pyogenes bacteria with different concentrations of the extracts given. Keywords: inhibition test, extract, Andrographis paniculata Nees, Moringa oleifera L., Streptococcus pyogenes
- Kasus infeksi dan resistensi antibiotik oleh bakteri Streptococcus pyogenes semakin meningkat. Daun sambiloto memiliki fitokimia unik yaitu andrographolide sebagai antibakteri serta fitokimia lainnya seperti alkaloid, flavonoid, phenol, saponin. Sedangkan daun kelor yang sering dikonsumsi sebagai sayuran memiliki aktivitas antibakteri dengan fitokimia meliputi vitamin C, alkaloid, flavonoid, fenolat, triterpenoid, dan tannin. Penelitian ini bertujuan untuk menganalisis daya hambat ekstrak etanol daun sambiloto, daun kelor, dan kombinasinya terhadap pertumbuhan bakteri Streptococcus pyogenes secara in vitro. Jenis penelitian ini adalah penelitian menggunakan metode true experimental post-test only control group design. Sampel dibagi menjadi 2 kelompok yaitu kelompok kontrol dan kelompok perlakuan dengan variasi konsentrasi 25%, 50%, 75%, dan 100%. Data dianalisis menggunakan aplikasi SPSS menggunakan metode Kruskal-Wallis dengan p<0,05. Hasil penelitian menunjukkan ekstrak etanol daun sambiloto tidak memiliki daya hambat terhadap Streptococcus pyogenes pada konsentrasi 25%, namun memiliki daya hambat tertinggi pada konsentrasi 100% yaitu 8,67 mm. Daya hambat esktrak etanol daun kelor tertinggi pada 100% yaitu 9 mm. Zona hambat yang dihasilkan oleh kombinasi ekstrak etanol daun sambiloto dan daun kelor menunjukkan efek sinergis pada konsentrasi 100% yaitu 9,33 mm. Kontrol positif yang digunakan amoxicillin dengan luas zona hambat 26,4 mm. Ekstrak daun sambiloto, daun kelor, dan kombinasinya memiliki daya hambat terhadap pertumbuhan bakteri Streptococcus pyogenes dengan perbedaan konsentrasi ekstrak yang diberikan. Kata kunci: uji daya hambat, esktrak, Andrographis paniculata Nees, Moringa oleifera L., Streptococcus pyogenes