AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KOPI ROBUSTA (Coffea canephora) TERHADAP BAKTERI Staphylococcus epidermidis ATCC 12228 PENYEBAB INFEKSI NOSOKOMIAL
| Main Authors: | Widyasari, Putu Ayu Melati, Aman, IGM, Mahendra, Agung Nova |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Universitas Udayana
, 2020
|
| Online Access: |
https://ojs.unud.ac.id/index.php/eum/article/view/71459 https://ojs.unud.ac.id/index.php/eum/article/view/71459/38827 |
Daftar Isi:
- Abstrak Infeksi nosokomial merupakan infeksi yang terjadi dikarenakan bakteri Staphylococcus epidermidis yang menyerang persendian dan pembuluh darah serta timbulnya pembengkakan atau abses, infeksi terlokalisasi pada kulit, saluran kemih, dan organ ginjal. Terdapat penelitian mengenai bakteri Staphylococcus epidermidis resisten terhadap antibiotika yaitu karbanesilin, sefuroksim, metronidazol dan sulfametoksazol/trimetasprim. Ekstrak biji kopi robusta (Coffea canephora) memiliki senyawa dengan efek antibakteri dan antioksidan yaitu kafein, asam klorogenat dan flavanoid. Tujuan penelitian ini yaitu untuk membuktikan biji kopi robusta (Coffea canephora) yang telah diekstrak dengan etanol dapat menghambat pertumbuhan bakteri.Staphylococcus epidermidis. Penelitian ini merupakan penelitian eksperimental in vitro dengan desain penelitian true experimental post test only control group design. Sampel dikelompok menjadi 5, yakni kelompok kontrol positif, kontrol negatif serta kelompok perlakuan dengan konsentrasi 10%, 50%, dan 100%. Dari penelitian diketahui bahwa ekstrak etanol dari biji kopi robusta (Coffea canephora) mampu menghambat pertumbuhan bakteri Staphylococcus epidermidis ATCC 12228 pada konsentrasi 50% dan 100% dengan rerata diameter zona hambat 6,8 mm sampai 9 mm. Sehingga disimpulkan bahwa ekstrak etanol dari biji kopi robusta menghambat pertumbuhan bakteri ini namun daya hambatnya lebih rendah dibandingkan kontrol positif. Kata Kunci: Kopi Robusta, infeksi nosokomial, Staphylococcus epidermidis
- Abstract Nosocomial infection is an infection caused by the bacterium Staphylococcus epidermidis which attacks joints and blood vessels and can cause swelling or abscesses such as zits, skin infections, urinary tract infections, and kidney infections. There is a study of 100% Staphylococcus epidermidis bacteria against carbanesilin, cefuroxime, metronidazole and sulfamethoxazole / trimetasprim. Robusta coffee extract (Coffea canephora) has compounds with antibacterial and antioxidant effects, namely caffeine, chlorogenic acid and flavonoids. The purposes of the study was to prove the ethanol extract from beans of Robusta coffee (Coffea canephora) can inhibit the growth of Staphylococcus epidermidis bacteria. This study was a laboratory experimental study in vitro using the true experimental method posttest only control group design. The sample was divided into 5 groups namely positive control group, negative control and treatment group with concentrations of 10%, 50%, and 100%. From the research it was known that the ethanol extract of robusta coffee beans (Coffea canephora) was able to inhibit the growth of Staphylococcus epidermidis ATCC 12228 at concentrations of 50% and 100% with the average diameter of inhibition zones of. 6.8 mm to 9 mm. The conclusion was the ethanol extract of robusta coffee beans can inhibit the growth of this bacterium but its inhibitory power was lower than positive control. Keywords: Robusta coffee, nosocomial infection, Staphylococcus epidermidis