VALIDASI METODE ANALISIS PENETAPAN KADAR AMOKSISILIN 500mG KAPLET SECARA KROMATOGRAFI CAIR KINERJA TINGGI
| Main Author: | Bakoh Sambodo, M. Caecilia N.Setiawati H |
|---|---|
| Format: | Article application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
STIFAR - Sekolah Tinggi Ilmu Farmasi
, 2013
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| Online Access: |
http://journal.stifar.ac.id/ojs/index.php/js/article/view/104 |
Daftar Isi:
- Analitical method for a concentration determination should give a valid result. That’s way it need an act to proof the acuration of that analitical method, with a validation. High Performance Liquid Chromatography (HPLC) is one of a method that use to determinate an active ingredient of a drug, such as the concentration of amoxicillin 500mg in a caplet. This research aims to proof the acuration of HPLC analytical method to determine the Amoxycillin content in caplet. Parameters of validation tested in this research were linearity, presision, acuration, robustness and conformity system test. This procedure validation used 10 ml KH2PO4 1 M and 1 ml CH3COOH 1 N dalam 1000 ml. as a solvent. The moving fase was aquadest : acetonitril : KH2PO4 1 M : asetic acid 1 N with proportion (909 : 80 : 10 1).standart solution and sample concentration were 70, 80, 90, 100, 110, 120, and 130 % and was measured according to the determination procedure of Amoxycillin tablet. The result of the test, point of correlation coefficient (r) for linearity were 0,9979 and 0,9964, relative standard deviation for pressision was 0,6 and 1,62%, acuration 1,96 and 1,78%, robustness 0,34 and 0,42%. Conformity system test for broad area was 1,50 retention time 0,09%, resolution 0,00% and recovery percentage for acuration 99,58%, robustness 98,71% and 100,94%. The result of validation parameters linearity, acuraty, pressicy, robustness and Conformity system test was compared with accept criteria, makes the high Performance Liquid Chromatography method was valid for determining amoxycillin500mg in a caplet form. Key Words : Validation, amoxicillin, High Performance Liquid Chromatography.