EVALUASI KERAGAMAN GENETIK TANAMAN KELAPA SAWIT (Elaeis guineensis Jacq.) DI KEBUN PERCOBAAN PPKS BERDASARKAN PRIMER SSR (Simple Sequence Repeats)
| Main Authors: | Aulia, Riski; Universitas Sumatera Utara, Siregar, Luthfi Aziz Mahmud; Universitas of Sumatera Utara, Bayu, Eva Sartini; University of Sumatera Utara, Setiowati, Retno Diah; University of Sumatera Utara |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Pasca Sarjana FP USU
, 2018
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| Subjects: | |
| Online Access: |
https://jurnal.usu.ac.id/index.php/tropik/article/view/20768 https://jurnal.usu.ac.id/index.php/tropik/article/view/20768/8831 |
Daftar Isi:
- This research aimed to obtain information of genetic diversity populations of E. guineensis using SSR markers. This research was conduted in Biology Moleculer Laboratory, Indonesian Oil Palm Research Institute, Marihat, Pematangsiantar, North Sumatera from March 2016 to November 2016. A total of 100 palm oil samples used in this research, including 20 samples from the populations of E. guineensis TR01S, including 20 samples from the populations of E. guineensis BJ022S, including 20 samples from the populations of E. guineensis BJ42S, including 20 samples from the populations of E. guineensis BO52S, and including 20 samples from the populations of E. guineensis MA19S. Calculation and Desciptive analysis in this research using software GeneMarker® version 2.40, software Power Marker V3.25, software NTSys version 2.1.1a and Microsoft Excell 2007. Research shows that the number of banding patterns detected 104 The banding patterns WITH range 8,7 per primary banding pattern, and the size of the banding patterns ranging from 101 bp -328 bp. mEgCIR2569 primary is the most widely primer to amplify DNA, the average of the percentage of polymorphism in the primer being used by 71% and the average value of . polymorficinformationcontent(PIC) 0,63. At 77% genetic similarity dendogram 100 samples split into 3 groups. Lowest genetic similarity value is 75% or the highest genetic diversity was 25%, while the value of the highest genetic similarity was 100% or the lowest genetic diversity is 0%.