Early Elucidation Of The Resistor Efficacy Of The Ethanol Ekstract Of The Leaf Of “Ketapang” (Terminalia Catappa L.) Against The Growth Of Staphylococcus Aureus ATCC25923 Which Is The Cause Of Gingivitist
| Main Authors: | Purwaningsih, Putu Pande, Darmayasa, Ida Bagus, Astiti, Ni Putu Adriani |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana
, 2020
|
| Online Access: |
https://ojs.unud.ac.id/index.php/metamorfosa/article/view/50426 https://ojs.unud.ac.id/index.php/metamorfosa/article/view/50426/34214 |
Daftar Isi:
- The leaf of “Ketapang” (Terminalia catappa L.) can be used as traditional medicine. The use of natural material is generally considered to be safer and has less side effect as compared to the use of modern medicine. This study aims at analysing the early elucidation of resistor property of the ethanol extract of the “ketapang” leaf against the growth of Staphylococcus aureus ATCC25923 as the cause of gingivitis, and to identify the active compound contained in the “ketapang” leaf. This study uses Complete Random Design (RAL) with 7 treatments of the extract concentration of “ketapang” leaf namely concentration of 1%, 2%,3%, 4%, 5%, positive control (chlorexidine) and negative control (ethanol). The discovery of the resistor efficacy of “ketapang” leaf extract against S. aureus bacteria using Kirby Beuer method has been indicated by the development of clear zone as the result of the application of anti-bacteria compound which is diffused in bacteria growth media. The finding of the study shows that the higher the concentration of the extract (5%), the wider the development of the zone of the resistor area. The effectiveness value of the anti-bacteria of “ketapang” leaf extract is not better than the antiseptic being tested. The result of profile test of phytochemical test shows that the extract of “ketapang” leaf contains tannin, saponin, terpenoid and flavonoid. The result of GC-MS test, it has been identified five compounds being contained in fraction IV namely Alpha Terpinolene, Cyclohexanol 5-methyl, Hexadecanoic acid, Octadecanoic acid, 1.2Benzenedicarboxylic acid. five active compounds that we found have the potentials of functioning as anti-bacteria.
- Daun Ketapang (Terminalia catappa L.) dapat dimanfaatkan sebagai obat tradisional. Penggunaan bahan alam sebagai obat secara umum dinilai lebih aman serta memiliki efek samping yang relatif lebih kecil dibandingkan penggunaan obat modern. Penelitian ini bertujuan untuk mengetahui elusidasi awal daya hambat ekstrak etanol daun ketapang terhadap pertumbuhan Staphylococcus aureus ATCC25923 penyebab gingivitis,dan untuk mengetahui senyawa aktif yang terdapat pada Daun Ketapang. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan konsentrasi ekstrak daun ketapang yaitu 1%, 2%,3%, 4%, 5%, kontrol positif (chlorexidine) dan kontrol negatif (etanol). Penentuan daya hambat ekstrak daun ketapang terhadap bakteri S. aureus menggunakan metode Kirby Beuer ditandai dengan terbentuknya zona bening akibat pemberian senyawa antibakteri yang berdifusi pada media tumbuh bakteri. Hasil Penelitian menunjukkan bahwa semakin besar pemberian konsentrasi ekstrak (5%) maka semakin besar zona hambat yang terbentuk.Nilai efektivitas antibakteri daun ketapang tidak lebih baik daripada antiseptik yang diujikan. Hasil uji profil fitokimia menunjukkan bahwa ekstrak daun ketapang mengandung tannin, saponin, terpenoid dan flavonoid. Hasil uji GC-MS teridentifikasi lima senyawa diantaranya yaitu Alpha Terpinolene, Cyclohexanol 5-methyl, Hexadecanoic acid, Octadecanoic acid, 1.2Benzenedicarboxylic acid. Kelima senyawa aktif tersebut berpotensi sebagai antibakteri.