Carbapenem Resistant Enterobacteriaceae Detection Methods
| Main Authors: | Kastawa, Ni Wayan Eka Putri Gayatri, Ramona, Yan, Fatmawati., N.N. Dwi |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana
, 2020
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| Online Access: |
https://ojs.unud.ac.id/index.php/metamorfosa/article/view/41986 https://ojs.unud.ac.id/index.php/metamorfosa/article/view/41986/34147 |
Daftar Isi:
- Carbapenem Resistant Enterobacteriaceae (CRE) is a group of Enterobacteriaceae resistant to Carbapenem antibiotics (Imipenem, Ertapenem, Meropenem, Doripenem). One species of Enterobacteriaceae frequently showing resistant characteristic to Carbapenem is Klebsiella pneumoniae. Early detection methods on the CRE existence (phenotypically or genetically) are urgently needed. The methods should be able to detect carbapenemases or genes encoding these types of enzymes. In the recent years, phenotypic characteristics can be done by applying methods, such as Modified Hodge Test (MHT), Carba Nordmann-Poirel (Carba NP), or Modified Carbapenem Inactivation Method (MCIM). To detect the presence of genes encoding production of carbapenemases, Polymerase Chain Reaction (PCR) is the most reliable method available in the recent years. Keywords: Carbapenem Resistant Enterobacteriaceae (CRE), Phenotype, Genothype, Modified Carbapenem Inactivation Method (MCIM), Modified Hodge Test (MHT), Carba Nordmann-Poirel, Polymerase Chain Reaction (PCR).
- Carbapenem Resistant Enterobacteriaceae (CRE) merupakan kelompok bakteri Enterobacteriaceae yang resisten terhadap antibiotik golongan Carbapenem (Imipenem, Ertapenem, Meropenem, Doripenem). Salah satu spesies Enterobacteriaceae yang sering menunjukkan sifat resisten terhadap Carbapenem adalah Klebsiella pneumoniae. Untuk mendeteksi keberadaan CRE perlu dilakukan deteksi dini menggunakan uji fenotip dan genotip. Uji fenotip yang dapat dilakukan adalah Modified Hodge Test (MHT), Carba Nordmann-Poirel (Carba NP), dan Modified Carbapenem Inactivation Method (MCIM). Untuk mengetahui keberadaan gen penyandi enzim carbapememase, metoda yang dapat dipakai dalam uji genotip adalah metoda Polymerase Chain Reaction (PCR) Kata kunci : Carbapenem Resistant Enterobacteriaceae (CRE), Fenotip, Genotip, Modified Carbapenem Inactivation Method (MCIM), Modified Hodge Test (MHT), Carba NP, Polymerase Chain Reaction (PCR).