Characterization of Protease from Bacillus licheniformis F11.1 as a Bio-Detergent Agent
| Main Authors: | Sitti Nur Ilmiah; Study Program of Biotechnology, Postgraduate School, Institut Pertanian Bogor, Bogor 16680, Nisa Rachmania Mubarik; Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor, Bogor 16680, Budiasih Wahyuntari; LAPTIAB 1, Agency for Assessment and Application of Technology, Science and Technology Research Center, Tangerang 15314 |
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| Other Authors: | Center for Bioindustrial Technology (BPPT) |
| Format: | Article application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Directorate of Research and Community Engagement, Universitas Indonesia
, 2018
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| Subjects: | |
| Online Access: |
http://journal.ui.ac.id/index.php/science/article/view/8809 |
Daftar Isi:
- Proteases are among the most important enzymes in both food and non-food industries taking up almost 60% of the world enzyme market. This enzyme has been used for many industrial processes, especially in the detergent industry. The purpose of this study was to characterize the protease from Bacillus licheniformis F11.1 as a bio-detergent agent. An enzyme assay of protease activity was used to assess and characterize the protease enzyme from B. licheniformis F11.1. It showed that the highest pH protease activity for alkaline protease occurred at pH 8.0 with a value of 35.00 U/mL. Under incubation temperature, the protease had the highest activity at 50 °C with a value of 24.46 U/mL. Protease activity was inhibited by Ca2+, Mn2+, K+, and Na+ ions at concentrations of 5 mM. Protease activity can be enhanced by these ions at concentrations of 2 mM. Protease stability can be measured from half-life. Under an incubation temperature of 50 °C, the half- life of the protease at pH 8, 9, and 10 was 108 min, 114 min, and 98 min, respectively. The assay for enzyme stability with an incubation temperature of 60 °C showed half-lives of 92 minutes, 56 minutes, and 61 minutes for pH 6, 9, and 10, respectively. This enzyme was found to be stable with the addition of detergent compounds such as sodium dodecyl sulfate (SDS), Triton X-100, ethylenediaminetetraacetic acid (EDTA), and hydrogen peroxide; all under low concentrations. Determination of the molecular weight using SDS- PAGE and zymogram found the molecular weight was 32.90-35.16 kDa. These results showed that the alkaline protease from B. licheniformis F11.1 can be used as a bio-detergent because of its tolerance to various detergent compounds.