Development of Immunochromatographic Strip Tests for Selective and Quantitative Detection of Melamine
| Main Authors: | Wiyogo P Wicaksono; Department of Chemistry, Faculty of Mathematics and Science, Universitas Indonesia, Depok 16424, Tribidasari A Ivandini; Department of Chemistry, Faculty of Mathematics and Science, Universitas Indonesia, Depok 16424, Endang Saepudin; Department of Chemistry, Faculty of Mathematics and Science, Universitas Indonesia, Depok 16424, Yasuaki Einaga; Department of Chemistry, Faculty of Science and Technology, Keio University 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522 |
|---|---|
| Format: | Article application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Directorate of Research and Community Engagement, Universitas Indonesia
, 2014
|
| Subjects: | |
| Online Access: |
http://journal.ui.ac.id/index.php/science/article/view/3722 |
Daftar Isi:
- An immunochromatographic strip test based on the complex reaction of antigen-antibody (melamine-antimelamine) was developed for quantitative detection of melamine. Gold nanoparticles (AuNP) were used to form AuNP-labeled antibody, which then acted as a biosensor. Melamine quantification was performed by the determination of AuNP using anodic stripping voltammetry technique with a boron-doped diamond as the working electrode. With sample volume of 100 µL and immunoreaction time of 7 min, the developed immunochromatographic strip test produced a linear calibration curve for melamine concentration range of 0–0.6 mg/L, with detection limit of 0.1 mg/L and RSD of ~5%. Furthermore, negative results were obtained for samples containing cyanuric acid and urea, indicating that the developed immunochromatographic strip test has potential for selective and quantitative detection of melamine.