PEMURNIAN DAN KARAKTERISASI XILANASE Streptomyces sp. SKK1-8
| Main Authors: | Anja Meryandini, Nunuk Widhyastuti, Yulin Lestari |
|---|---|
| Format: | Article application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Directorate of Research and Community Engagement, Universitas Indonesia
, 2010
|
| Online Access: |
http://journal.ui.ac.id/index.php/science/article/view/336 |
Daftar Isi:
- Purification and Characterization of Streptomyces sp. SKK1-8 xylanase. Streptomyces sp. SKK1-8 is a xylanase produced bacteria. Purified xylanase has an optimum condition at pH 4.5 and 50oC. The molecular mass of purified xylanase were determined to be 14.4 kDa and 13.4 kDa. The xylanase was capable of hydrolysing p-NP-α-L-arabinofuranoside, p-NP-β-D-xylanopiranoside, p-NP-β-D-glucopiranoside, p-NP-α-D-galactopiranoside. The Km and Vmax values at 50oC measured on Birchwood xylan were 0.101 mg/ml and 0.1796 μmoles/minute/ml. Keywords: Xylanase, streptomyces, purified