Isolation and Identification of Airborne Bacteria Inside Swiftlet Houses in Sarawak, Malaysia
| Main Authors: | Leong Sui Sien; Department of Molecular Microbiology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia, Chia Hwa Chuan; Department of Molecular Microbiology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia, Samuel Lihan; Department of Molecular Microbiology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia, Ling Teck Yee; Department of Chemistry, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia |
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| Format: | Article application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Directorate of Research and Community Engagement, Universitas Indonesia
, 2014
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| Subjects: | |
| Online Access: |
http://journal.ui.ac.id/index.php/science/article/view/2950 |
Daftar Isi:
- Abstract Air consists of such microorganisms as bacteria, fungi, and viruses. Exposure to these airborne bacteria indoors may cause infectious and noninfectious adverse health effects. However, the sources and origins of bacteria are not fully understood. The aim of this study was to isolate and identify the bacteria present in the air inside swiftlet houses located in Kota Samarahan, Saratok, Betong, Maludam, Miri, Kuching, Semarang, Sepinang, Sarikei, and Sibu in Sarawak, Malaysia. A total of 100 bacterial isolates from 20 samples were collected from swiftlet houses. The bacteria present in the air were collected using Plate Count Agar. Two plates were exposed at the front and back inside swiftlet houses for 15 sec and then incubated at 37 ± 1 oC for 24 h. The 16S rRNA analysis method was used to identify the isolates from the samples. The air inside the swiftlet houses had a total mean airborne bacteria colony count of 2.02 ± 0.72 log10 cfu/m2/sec; the highest was in Miri (3.08 ± 0.29 log10 cfu/m2/sec), and the lowest was in Sibu (1.05 ± 0.85 log10 cfu/m2/sec). Twenty-seven bacteria species were identified, and Lysinibacillus sp. B4 (16%) was most frequently isolated. Abstrak Isolasi dan Identifikasi Bakteri yang terdapat di Udara dalam Rumah Walet di Sarawak, Malaysia. Udara mengandung mikroorganisme seperti bakteri, jamur , dan virus. Paparan bakteri di udara dapat menyebabkan efek kesehatan yang merugikan. Namun, sumber dan asal bakteri tidak sepenuhnya dipahami. Tujuan dari penelitian adalah mengisolasi dan mengidentifikasi bakteri yang terdapat di udara di dalam rumah-rumah walet yang terletak di Kota Samarahan, Saratok, Betong, Maludam, Miri, Kuching, Semarang, Sepinang, Sarikei dan Sibu di Sarawak, Malaysia. Sebanyak 100 isolat bakteri dari 20 sampel diperoleh dari rumah-rumah walet. Bakteri diisolasi menggunakan medium Plate Count Agar. Sebanyak dua cawan petri dibuka di bagian depan dan belakang dalam rumah walet selama 15 detik, dan diinkubasi pada suhu 37±1 oC selama 24 jam. Sequencing DNA gen 16S rRNA dilakukan untuk mengidentifikasi bakteri dari sampel. Udara di dalam rumah walet memiliki total rata-rata jumlah koloni 2.02±0.72 log10 cfu/m2/detik dan CFU tertinggi diperoleh dari Miri (3.08 ± 0.29 log10 cfu/m2/detik) dan terendah di Sibu (1.05 ± 0.85 log10 cfu/m2/detik). Terdapat 27 species bakteri dari udara dalam ruangan di rumah walet, dan Lysinibacillus sp. (16%) paling banyak ditemukan. Keywords: Swiftlet house, Air samples, Bacteria, Identification, 16S rRNA gene