Isolation and Identification of Fenolic Acid in Katu Leaves (Sauropus Androgynus (L.) Merr. )
| Main Author: | Sri Harsodjo Wijono Soewandi; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, National Institute of Science and Technology, Jakarta 12640 |
|---|---|
| Format: | application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Directorate of Research and Public Services Universitas Indonesia
, 2010
|
| Subjects: | |
| Online Access: |
http://journal.ui.ac.id/index.php/health/article/view/275 |
Daftar Isi:
- To improve traditional drugs in changing to fitofarmaka studies should be conducted on the chemical content of plants used as drugs. In this study, fenolic acid compounds in katu leaves, which are used for traditional medicine, were isolated and identified. From 95% ethanol extracts of katu leafs could be isolated a fenolic acid compound through continuous fraction using ether, with or without hydrolysis process. The ether fraction was then separated with the two dimension paper chromatography. The first solution developed was 2% acetic acid in water, and the second was benzene–acetic acid–water (60 : 22 : 1,2). Spots were identified with ultraviolet light, diazo p-nitroaniline solution and to enhance the color 15% sodium carbonate was sprayed. After separation p-hidroxy benzoid acid, ferulic acid, cafeic acid and vanilic acid were identified. In addition more than 7 spots were found which were supposed to be fenolic acids. Quantitative analysis was done using the spectrophotodensitometer for 4 kinds of identified fenolic acids. The highest percentage in katu leafs was p-hydroxibenzoid acid.