ANALISIS 8-ISOPROSTAN DALAM URIN TIKUS JANTAN WISTAR SETELAH TERPAPAR ETANOL DAN ASAP ROKOK
| Main Authors: | Ari Chandra Wibawa, Agung, Suaniti, Ni Made, Ariati, Ni Komang |
|---|---|
| Format: | Article application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Journal of Chemistry
, 2014
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| Subjects: | |
| Online Access: |
http://ojs.unud.ac.id/index.php/jchem/article/view/9747 |
Daftar Isi:
- The 8-isoprostane compound can be used for a biomarker of oxidative stress formed by lipid peroxidation in the form of arachidonic acid with free radical in the body. The purpose of this study was to analysis urinary 8-isoprostane in male Wistar rats after sub-acute exposer to ethanol and cigarette smoke. Extraction method used was solid phase extraction and the analysis was conducted by TLC and spectrophotodensitometry. The results showed that the 8-isoprostane compound was not detected in the male Wistar rat’s urine as control rats and in those exposed to cigarette smoke. The average concentrations of 8-Isoprostane in the male Wistar rat’s urine exposed to ethanol and combination of ethanol-cigarette were 1.519 ± 1,386 and 2.063 ± 1,308 ppm respectively.